Translational_Unit

Part:BBa_K812057:Design

Designed by: Hafez El Sayyed   Group: iGEM12_Evry   (2012-09-26)


A GoldenBricked version of sfGFP protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 16
    Illegal SpeI site found at 754
    Illegal PstI site found at 760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4
    Illegal SpeI site found at 754
    Illegal PstI site found at 760
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 16
    Illegal SpeI site found at 754
    Illegal PstI site found at 760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4
    Illegal XbaI site found at 16
    Illegal SpeI site found at 754
    Illegal PstI site found at 760
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 10
    Illegal BsaI.rc site found at 748
    Illegal SapI.rc site found at 33


Design Notes

GOlden Brick Assemply Proposed by our team


Source

This DNA sequence was acquired from Alfonso Jaramillo's Lab

References